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vol. 19, nr. 2 (2012)
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Interrelations between DNA concentration and physicochemical parameters in the loess soil profile
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Agnieszka Wolińska, Zofia Stępniewska, Agnieszka Wołoszyn, Miriam Rzewuska
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Department of Biochemistry and Environmental Chemistry, Institute of Biotechnology, The John Paul II Catholic University of Lublin, Al. Kraśnicka 102, 20-718 Lublin |
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vol. 19 (2012), nr. 2,
pp. 437-448
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abstract:
The purpose of presented study was determination of the impact of selected physicochemical parameters, strongly influencing the soil biological life: water potential (pF), oxygen availability for microorganisms (ODR), redox potential (Eh), content of Mg, Ca and total organic carbon (TOC) on soil DNA concentration. Undisturbed loess soil material was taken to metal cylinders (100 cm3) from four depths (0-20; 20-40; 40-60 and 60-80 cm), what make possibility for description of DNA content and its distribution in the whole soil profile. Our results revealed significant (p<0.05) positive relationships between soil DNA content and measured values of ODR (r = 0.94***), Eh (r = 0.52**) and TOC (r = 0.98***), what were confirmed by high values of correlation coefficients (r). Whereas, significant negative interrelationships between soil DNA and pF (r = –0.57*) or Mg content (r = –0.79***) were determined. However, in the current experiment conditions, we did not found significant correlation between Ca presence and DNA content (p>0.05). Significant (p<0.05) decrease of DNA concentration by 62.8% with an increase of soil depth was noted, what was undoubtedly connected with spatial distribution of microorganisms in the soil profile and its likes for surface layers colonization.
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keywords:
DNA content, soil water potential, oxygen availability, redox potential, divalent cations
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original in:
English
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