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vol. 4, nr. 1 (2004)
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The effect of pre-sowing laser light treatment on some biochemical and physiological processes in the seeds and plants of white lupin and faba bean
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Janusz Podleśny1, Anna Stochmal2
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1 Department of Forage Crop Production, The Institute of Soil Science and Plant Cultivation ul. Czartoryskich 8, 24-100 Puławy |
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2 Depatment of Biochemistry and Crop Quality, The Institute of Soil Science and Plant Cultivation ul. Czartoryskich 8, 24-100 Puławy |
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vol. 4 (2004), nr. 1,
pp. 149-160
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abstract:
The studies were conducted at the Department of Forage Crop Production of the Institute of Soil Science and Plant Cultivation (IUNG) in Puławy, and laboratory analyses of seeds and seedlings at the IUNG Department of Biochemistry and Crop Quality. Free radicals in seeds using the EPR method were determined at the Maria-Curie Skłodowska University in Lublin. The studies included faba bean of the Tom variety and white lupine of the Butan variety. The seeds were exposed to a helium-neon laser beam at 3 dosage levels: D0 � no exposure, D3 � 3 exposures, D5 � five exposures. The dose during a single exposure was 4Ľ10-3 J cm-2 s-1. Pre-sowing treatment of sowing material with laser beams increased considerably the activity of amylolytic enzymes in white lupine and faba bean seeds. The greatest diversification of the enzymatic activity in seeds which were exposed and not exposed to laser light treatment was noticed after 96 hours from the day of sowing. The activity of the examined enzymes in the seeds of both examined plant species was similar and they had a similar course in time. The exposed seeds of white lupine and faba beans had greater weight at the time of swelling than the seeds which were not exposed. It resulted in earlier and more even germination. It was noticed that the concentration of free radicals increased considerably in the seeds pre-treated with the laser beam. The largest increase of free radicals in sowing material of both plant species was noticed after three exposures of the seeds. Pre-sowing biostimulation with a laser had a positive influence on the growth and development of the seedlings which grew from them. Lupine and faba beans seedlings which grew from exposed seeds had in subsequent measuring periods longer hypocotyl and roots when compared to seedlings which grew from seeds not exposed.
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keywords:
biostimulation of seeds, enzymes activity, free radicals, germination, helium-neon laser
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original in:
Polish
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