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vol. 5, nr. 2 (2005)
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Laser light as a physical factor enhancing rapeseed resistance to blackleg disease
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Michał Starzycki1, Wojciech Rybiński2, Eligia Starzycka1, Jan Pszczoła3
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1 Plant Breeding and Acclimatization Institute, Poznań Department, ul. Strzeszyńska 36, 60-479 Poznań |
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2 Institute of Plant Genetic of Polish Academy of Sciences, ul. Strzeszyńska 34, 60-479 Poznań |
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3 Plant Breeding Company Strzelce, Borowo Department, 64-020 Czempiń |
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vol. 5 (2005), nr. 2,
pp. 441-446
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abstract:
The history of hardening crop plants by laser light is relatively short. After previous experimental confirmation of positive influence of laser light on seed condition, an attempt was made to use it to enhance winter rapeseed resistance to the most dangerous fungal pathogen – Phoma lingam (Tode ex Fr.) Desm. Such characteristics of laser beam as power, wavelength, and exposure time are of great importance for the stimulating effect. Inappropriate parameters can even cause irreparable mutation changes on the DNA level. At present, controlled seed irradiation with laser light finds practical application in seed stimulation more and more often. In this study, three different forms of winter rapeseed were used: two open pollinated varieties – Bolko and Idol, and a yellow-seeded strain (IHAR-2061/03). The seeds were irradiated with helium-neon laser at wave-length of 632 nm. The intensity of light falling on the objects was 1 mW cm-2. Four exposure times were used: 30, 60, 90, and 120 min. Non-irradiated seeds were used as the control. Mycelium test was applied to check which combination had the most stimulating effect. After plant inoculation, the best results for the yellow-seeded form and variety Idol were obtained for exposure times of 30 and 60 min, and for variety Bolko – 30, 60 and 90 min. The results of this work can be used in breeding as well as in seed production to enhance rapeseed rape mechanisms of resistance to one of the principal diseases affecting this crop.
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keywords:
disease resistance stimulation, laser, Phoma lingam, winter rapeseed
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original in:
English
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