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vol. 10, nr. 1 (2007)
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Regeneration of Miscanthus x giganteus calli with use of stimulation effect of helium-neon laser light
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Katarzyna Głowacka, Wojciech Rybiński, Stanisław Jeżowski
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Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszyńska 34, 60-479 Poznań |
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vol. 10 (2007), nr. 1,
pp. 79-88
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abstract:
In 1935 the genus Miscanthus was introduced, by the Danish traveller Olsen, from Japan to Europe. In the beginning grasses from that genus were treated as ornamental plants because of their attractive inflorescence. Since the year 1982 Miscanthus x giganteus, and also Miscanthus sinensis, have been seen as an alternative source of energy. Very effective photosynthesis with high biomass growth in time unit per assimilation area is what causes those perennial Miscanthus plantations to be a potential renewable ecological source of energy. The experiments were a part of the research work on micropropagation methods improvement and polyploidization in cultures in vitro of Miscanthus species. The present experiments were performed to evaluate the effect of laser light on the Miscanthus x giganteus calli regeneration. In the research a helium-neon laser was used, prepared by The Laser Technology Centre in Warszawa, which emits red light of the wave-length of 632 nm with 24 mW power and power density of 1 mW cm-2. The embryogenic, three-month old calli from inflorescence fragments were irradiated with laser light. The He-Ne laser light had a stimulated effect on shots regeneration from irritated calli, while there was not observed any clear laser effect on the number of regenerated calli. A slight reduction of shoots length was also observed. The use of laser increased the number of regenerated shoots by 26.1-30.1% in comparison to the control. The biostimulation effect was also observed during further growth of Miscanthus x giganteus plants in greenhouse conditions.
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keywords:
calli, helium-neon laser, in vitro culture, Miscanthus x giganteus, stimulation effect
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original in:
Polish
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