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vol. 18, nr. 2 (2011)
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Control of natural mycobiota in maize grains by ultraviolet (UVC) irradiation
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Carmen Liliana Rodríguez Páez1, María Cristina Pérez Reyes2, Claudia Hernández Aguilar1, Flavio Arturo Domínguez Pacheco1, Ernesto Moreno Martínez2, Alfredo Cruz Orea3, José Luis López Bonilla1
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1 Instituto Politécnico Nacional, SEPI-ESIME, Zacatenco, Unidad Profesional “Adolfo López Mateos”, Delegación Gustavo A. Madero, México, D.F. C.P. 07738 |
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2 Unidad de Investigación en Granos y Semillas FESC, UNAM |
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3 Departamento de Fisica, Cinvestav-IPN, AP-14 740, Mexico D.F. CP 07360, Mexico |
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vol. 18 (2011), nr. 2,
pp. 375-388
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abstract:
The effect of UV-C light as a means of control of natural mycobiota of grains of maize (Zea mays L.) hybrids “San Juan” and ”H-159” (productive cycle, 2009) was investigated. UV-C lamps of 15 W were used and the exposure times applied were 0, 5, 10, 15, 20, 25 and 30 min. The experiment was established in the randomised complete block design with eight and four replicates. The unit pilot was 50 grains. For the determination of mycobiota the agar plate test was used, after disinfection of the grains with sodium hypochlorite diluted to 3% for 1 min. Differences (P ≤ 0.001, P ≤ 0.05) between treatments were found, the best treatments being those of 30 and 10 min, observing reductions of 42.85 and 52.05% in the number of grains infected with Fusarium spp. with respect to control for “San Juan” and “H-159”, respectively. For Fusarium monoliforme it was found that in 30 min there was a reduction of 53.74% for the hybrid “San Juan”, while for H-159 a reduction of 61.7% in 10 minutes was observed. These results show that UV-C radiation may be useful for application as a germicide in future experiments on a wide variety of grains.
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keywords:
Zea mays, ultraviolet radiation, maize grain, fungi, mycobiota
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original in:
English
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