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vol. 19, nr. 1 (2012)
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Methanotrophs responsible for methane oxidation in natural peats from Polesie Lubelskie Region
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Anna Szafranek-Nakonieczna, Zofia Stępniewska, Agnieszka Wołoszyn, Jakub Ciepielski
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Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University, Al. Kraśnicka 102, 20-718 Lublin, Poland |
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vol. 19 (2012), nr. 1,
pp. 181-193
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abstract:
The potential of methanotrophic activity (MTA) has been investigated under laboratory conditions in three types of peatland profiles: high (H), transition (T) and low (L) originating from Polesie Lubelskie Region. Selected peat samples differed in respect of pH, TOC, von Post index and moisture. The experiment was conducted at natural moisture (198-719 %w/w) with different ranges of both, temperature (5, 10 and 20°C) and CH4 enrichment (1 and 5%v/v). The highest MTA (19.69-155.79 mg CH4kg D.W.-1 d-1) was observed at 20°C. Regardless of temperature, MTA was lower (1.38-51.16 mg CH4 kg D.W.-1 d-1) when peat samples were incubated in atmosphere enriched in 1% than in 5% CH4 v/v (4.75-191.26 mg CH4kg D.W.-1 d-1). Strong influence of temperature and sampling sites on MTA was also noted. Total DNA was isolated from the most active (20°C, 5% CH4 v/v) peat samples from each site and the PCR (polimerase chain reaction) amplifying of genes pmoA (primers A189f/mb661r) and sequence 16S rRNA (primers Type If /Type Ir and Type IIf/Type IIr) specific for methanotrophic bacteria were carried out. Positive results of PCR with primers of pmoA gene after sequencing confirmed that methanotrophs from L point belong to family Methylococcaceae, while 16S rRNA gene sequences from microorganisms inhabiting H peat demonstrated the highest similarity to genus Methylocystis and Methylosinus.
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keywords:
methanotrophic activity, methanotrophs, peat soil
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original in:
English
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