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vol. 2, nr. 3 (2003)
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Influence of helium-neon laser on androgenesis in some chosen varieties of winter triticale
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Agnieszka Katańska1, Wojciech Rybiński2, Zbigniew Broda1
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1 Department of Genetics and Plant Breeding, University of Agriculture, ul. Wojska Polskiego 71c, 60-625 Poznań |
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2 Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszyńska 34, 60-479 Poznań |
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vol. 2 (2003), nr. 3,
pp. 559-566
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abstract:
Anthers of winter triticale of Tornado, Bogo, Sekundo, Kitaro, Kazo, Hewo and SZDB 154 breeding lines served as an initial material for the present research. In the experiment, the helium-neon laser operating in a red light range with a wavelength of 632 nm was applied. Anthers used in in vitro cultures were selected from the plants obtained from the grains irradiated with laser light and control plants (not irradiated). A part of control plants was used in the second variant in which anthers were irradiated right before placing them on a medium. Anthers from both variants of laser light irradiation were grown in an in vitro culture and the processes of androgenesis were analyzed. The number of callus obtained per 100 plated anthers indicated a biostimulating activity of laser light as compared to combinations without irradiation (control). This effect was visible in the case of plated anthers derived from the plants with grain irradiation as well as from directly irradiated anthers. The number of albino plants was higher in the combinations with laser light irradiation but no synonymous effect was observed for the number of green plants obtained.
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keywords:
androgenssis, anthers, biostimulation, laser, triticale
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original in:
Polish
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